UN spokesman Stephane Dujarric in New York defended the United Nations Assistance Mission in Afghanistan, or UNAMA, and its head Roza Otunbayeva, who said the new laws provided a "distressing vision" of Afghanistan's future.
She said last week the laws extended the "already intolerable restrictions" on the rights of women and girls, with "even the sound of a female voice" outside the home apparently deemed a moral violation.
The laws were issued after they were approved by supreme leader Hibatullah Akhundzada.
The Taliban had set up a ministry for the "propagation of virtue and the prevention of vice" after seizing power in 2021.
They say the laws are based on their interpretation of Sharia, or Islamic law.
The ministry called on international organisations, countries and individuals to respect the religious values of Muslims.
It announced on Friday it would no longer co-operate with UNAMA because of its criticism of the laws.
"We have been very vocal on the decision to further make women's presence almost disappear in Afghanistan.
"In terms of the contacts with the de facto authorities, I mean, we will continue to engage with all stakeholders in Afghanistan, including the Taliban," Dujarric said at a news conference.
"We have always done so following our mandate and I would say impartially and in good faith, always upholding the norms of the UN, pushing the messages of human rights and equality.
"And we will continue our work as mandated by the Security Council."
