Giuffre, also known as Virginia Roberts, is suing the Duke of York for damages in her home country of the US, claiming she was trafficked by Jeffrey Epstein, Andrew's friend, to have sex with the royal when she was 17, a minor under US law.
It is understood the agreement for Giuffre to be questioned by the duke's lawyers, Andrew Brettler and Melissa Lerner, has now been struck and an exact date has been confirmed.
Andrew will also give evidence under oath on March 10 in London, in what has been described as a neutral location.
The duke's "deposition" will be conducted by Giuffre's lawyers, David Boies and Sigrid McCawley, and is reportedly expected to last two days.
Andrew is alleged to have sexually abused Giuffre at Ghislaine Maxwell's London home, during a visit to Epstein's private island Little St James and on a separate occasion at the financier's Manhattan mansion - all in the early 2000s.
Andrew has strenuously denied all allegations.
Last month, court documents revealed that US Judge Lewis A Kaplan had written letters to the Senior Master of the Queen's Bench Division in the UK to request assistance in acquiring evidence for the civil claim filed by Giuffre.
The judge asked for the royal's former assistant Robert Olney to be quizzed on topics such as Andrew's communications with Maxwell, Epstein and Giuffre and his travel to Epstein's homes.
In a separate letter to the High Court, Judge Kaplan also requested testimony from Shukri Walker, a witness who says she saw Prince Andrew with a "young girl", on questions about the alleged presence of the duke and Giuffre at London's Tramp nightclub in March 2001 and any interactions the pair had.
The civil sex assault trial in the US is scheduled to take place between September and December.
The parties will need to confirm by July 28 whether they wish to proceed to trial.
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