The operation will run for five days from 12.01am on Friday, November 1 until 23.59pm on Tuesday, November 5.
Police will be enforcing road rules across the state to reduce harm on the roads and to positively influence road user behaviour.
This year, the focus will be on impaired driving, fatigue, distraction offences, seatbelt non-compliance, vulnerable road user safety and speeding.
Echuca police Acting Sergeant Gary Atkins said there would also be zero tolerance for those who drive under the influence.
“There will be zero tolerance for drink and drug driving over the Melbourne Cup Carnival long weekend, and motorists should expect to be tested while out on the roads,” he said.
“If you’re planning to have a drink on Melbourne Cup Day, don’t drive – leave the car at home, arrange a designated driver and utilise alternative transport.”
During last year's Operation Furlong, 11 lives were lost during the Melbourne Cup weekend.
Victoria Police also recorded 8373 traffic infringements, including 287 drink-driving offences and 176 drug-driving offences.
This year, police will once again be deployed at high-risk areas across the state, focusing on major routes linking Melbourne to popular holiday destinations.
Sgt Atkins added that police would be targeting those driving on high-risk rural roads and highways.
“Police will be targeting speeding and distracted drivers heading to and from regional areas on high-risk rural roads and highways,” he said.
“Slow down, be patient and be courteous to other motorists.”