She joins a large crossbench of 11 minor party upper house members, with four members of the Greens and two from the Legalise Cannabis Party.
Ms Tyrrell has previously run in state and federal elections for the lower houses of parliament, but ran for a place in the upper house for this election.
She stands to be the sole One Nation member in Victorian Parliament following last month’s elections and will join Shepparton’s Wendy Lovell in the Legislative Council.
Attorney-General Jaclyn Symes, from Labor, was elected alongside Gaelle Broad, from the Nationals, and the Animal Justice Party’s Georgie Purcell.
Ms Tyrrell runs a dairy farm alongside her husband, Aaron.
The count was finalised on Wednesday, December 14 by the Victorian Electoral Commission.
One Nation’s national leader, Pauline Hanson, welcomed Ms Tyrrell to parliament, making Victoria the fourth state with a One Nation MP.
Ms Hanson said Ms Tyrrell had been a “strong advocate for water policies to give farmers more certainty and better water security”.
“For too long, Victoria’s irrigators have borne the brunt of water reform in the Murray-Darling Basin, and the cost of water has driven many farmers out of the industry,” Ms Hanson said.
Former Shepparton mayor Kim O’Keeffe, of the Nationals, was elected in the lower house for the seat of Shepparton.
Labor won 15 of the 40 upper house seats, meaning it will need six crossbench votes in order to pass legislation, while the Coalition nabbed 14 seats.