The national broadcaster's Southbank office in Melbourne's CBD had its front window daubed with "Tell the truth about Palestine" in white paint overnight and red paint was splashed on the building's doors.
Police have opened an investigation into the "criminal damage incident" at the commercial premises.
"Officers were called to reports unknown offenders had spray painted sections of the building on Southbank Boulevard about 3.50am," a Victoria Police spokeswoman said.
An ABC spokesman confirmed no one was hurt in the incident.
"There was minimal damage," he said.
The outside of the ABC offices in Melbourne have been splashed with red paint. (Joel Carrett/AAP PHOTOS)
Intergenerational conflicts in the Middle East region involving Palestinians and Israelis escalated on October 7 when Hamas militants attacked southern Israel from Gaza.
More than 1200 people died and up to another 200 were taken hostage, according to the United Nations.
Israel's subsequent military action has resulted in the deaths of around 20,000 people in Gaza, with the UN citing unconfirmed data provided by the local ministry of health and Gaza Government Media Office.
It's the second time in a week the offices were targeted with portraits of Palestinian journalists killed in the conflict plastered to the national broadcaster's office on Wednesday.
About 40 portraits were stuck to the ABC's office windows and doors depicting the names and sketches of the dead journalists.
Nine's Melbourne newspaper The Age and TV office also faced protests on Wednesday after activists blocked the entrance to the headquarters in Docklands.
The group, who were behind fake corpses placed outside seven Labor MPs' offices in November, donned press vests to protest the deaths of 95 journalists in Gaza, alleging "silence" from Australian media outlets.
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