The Australian Education Union has been trying to negotiate a new enterprise agreement for more than two years without success.
Following statewide classroom walkouts in June and July, unionised TAFE teachers will go on strike for 24 hours on Wednesday and rally outside the Victorian parliament.
TAFE teachers in the state last stopped work for 24 hours in 2012 to protest cuts, having previously gone on strike all day in 2008 as part of protected strike action.
The union's Victorian branch president Meredith Peace said the action was the result of the repeated refusal by the Allan Labor government to offer decent pay and conditions for TAFE teachers.
Meredith Peace says Victorian TAFE teachers are among the lowest paid in Australia. (James Ross/AAP PHOTOS)
"It is unacceptable that Victorian TAFE teachers are among the lowest paid in the nation," she said.
"TAFE teachers are burning out due to excessive and unsustainable workloads and too many are leaving the sector to return to industry, where they can be paid more without the added stress and heavy workloads they're currently experiencing.
"To make matters worse the state Labor government has broken its promise to 'save TAFE' and have been responsible for vocational education and training in Victoria being the lowest funded in Australia every year for the last 10 years."
The union is negotiating with the Victorian TAFE Association but the state government funds enterprise agreements.
Skills and TAFE Minister Gayle Tierney said she valued TAFE teachers' role in training the workers society needed.
"We recognise the right of unions and workers to take protected industrial action and hope that parties will continue negotiations," she said.
"We expect a swift resolution and urge both parties to compromise for a mutually beneficial outcome."
A recent report by the Victorian auditor-general showed the sector would have posted a $35 million deficit if it wasn't for government grants in 2023.