The state is poised to remain mostly dry on Wednesday with the flood focus shifting towards Murchison and Shepparton, and later to Echuca.
Authorities performed six more flood rescues in the 24 hours to 7am on Wednesday, bringing the total since the emergency began on Sunday to 52.
About half of those were triggered by people driving on flooded roads and becoming stuck in their cars, an State Emergency Service spokesperson told AAP.
Residents in warning zones are being encouraged to make sure they have sufficient supplies. (Diego Fedele/AAP PHOTOS)
A watch and act alert is in place along the Goulburn River from Murchison and Shepparton to south of Lake Eildon.
Victoria SES chief officer Tim Wiebusch earlier warned communities along the Goulburn River to expect possible road closures and overland flooding.
The river at the rural village of Murchison was slated to reach a moderate-level peak of 10.5m late on Wednesday morning, while moderate flooding was possible at Shepparton from Thursday morning.
From Kialla to Shepparton, up to 20 properties might be inundated with above-floor flooding and a further 150 properties could have their land or surrounding streets flooded, Mr Wiebusch said.
"Now is the time to prepare," he said on Tuesday afternoon.
"Make sure you have up to three days supplies so that you can, if you do become isolated, work through that period."
He said minor flooding was expected at Echuca, along the Murray River, later in the week.
More than 1800 requests for assistance have been made to Victoria's SES during the emergency. (Diego Fedele/AAP PHOTOS)
The Goulburn River at Seymour peaked at 6.8m on Monday afternoon before a second rise late on Tuesday.
The SES said six homes were flooded in Yea, eight to 10 houses in Seymour had above-floor flooding and a further 140 properties experienced other flooding, with crews set to examine the damage.
More than 1800 requests for assistance have been made to the SES during the emergency, but call numbers dropped over the last day as the response shifts towards recovery in many areas.
Rochester residents have been warned it's not yet safe to return, with a watch and act in place for the Campaspe River as SES volunteers continue taking stock of the damage.
The owner of an empty kayak seen floating down the Mitchell River near Dargo on Tuesday was later found safe and well in the area.
Mount Dandenong Tourist Road at Ferny Creek in Melbourne's southeast remains closed after a landslide measuring 70 by 40 metres.