Rallen Australia says Tamboran Resources has also started clearing land "dangerously close" to an Aboriginal sacred site on Tanumbirini Station.
"Tamboran has shown itself time and time again to take a cavalier approach, ignoring concerns about damage to vegetation, cattle, water safety and the many sacred sites on Tanumbirini," Rallen director Luciana Ravazzotti Langenhoven said on Wednesday.
The cattle baron claims Tamboran Resources, through its subsidiary Sweetpea Petroleum, is the first company to force its way onto a cattle station since the NT rolled out amended petroleum regulations in 2021 requiring parties to reach a land access agreement before operations started.
Rallen has also accused Sweetpea workers of bringing contaminated equipment onto the site that threatens the station's biosecurity.
"We're being forced to manage our cattle station while also closely monitoring how Tamboran conducts its fracking works so we can act on breaches of a regulatory regime," Ms Ravazzotti Langenhoven said.
Sweetpea recently won the legal right to explore parts of the 5000 sq km property in the gas-rich Beetaloo Basin, 600km southeast of Darwin, and previously informed Rallen it would be starting work.
But the pastoral company has not consented to Sweetpea's plans or the recent clearing of land outside of approved areas and the destruction of its fence along the Carpentaria Highway.
Legal letters sent by Rallen's lawyers demand Sweetpea install a stock-proof gate and a cattle grid to stop cattle wandering onto the busy roadway.
The company also raised concerns about Sweetpea's failure to comply with its own weed management plan by transporting contaminated equipment onto the station.
"The government has effectively forced us to allow Tamboran to enter Tanumbirini," Ms Ravazzotti Langenhoven said.
"There are literally zero government resources spent monitoring the gas industry's conduct, this gives this controversial company a free pass to wreak damage."
Rallen has also alleged that Tamboran doesn't have an approved environmental management plan after it withdrew its approved plans following complaints it had not properly consulted stakeholders.
Tamboran has previously said it would continue to defend its legal rights to explore the station.
"(We) attempted to engage in good faith negotiations with Rallen Australia to negotiate an equitable access agreement," a spokesman said.
"Tamboran has a legal right to access the EP 136 asset to explore for gas under the approved access agreement issued by the NT Civil and Administrative Tribunal.
"This approved access agreement was issued according to the careful and fair processes laid out in the NT's new Petroleum Regulation."
The company also says it continues to work closely with all stakeholders, including pastoral leaseholders and the Northern Land Council.
It has also committed to implementing all 135 recommendations to minimise the risks associated with an onshore shale gas industry that came from the 2018 scientific inquiry into hydraulic fracturing in the NT.
Rallen will challenge the NTCAT's decision to grant Tamboran Resources access in NT Supreme Court on June 20.
Rallen is one of Australia's largest landowners with six cattle stations and 70,000 head of cattle in the NT, which it has spent $200 million over the past four years developing.
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