The party was voluntarily deregistered following the 2022 federal poll and under commonwealth laws, is unable to re-register until after the next election.
Mr Palmer, his Victorian UAP Senator Ralph Babet and the party's national director Neil Favager launched a High Court bid to reverse the party's de-registration in 2024.
They argued the decision would impair voters' ability to directly elect UAP candidates and discriminated against the party's candidates.
Lawyers for the party also argued the prevention infringes on freedom of political communication.
But the panel of High Court justices unanimously ruled against Mr Palmer and Senator Babet on Wednesday, ordering them to pay the court's costs in the process.
The court will detail its reasons for the decision in due course.
Mr Palmer voluntarily deregistered his party in 2022 following the federal election, despite Senator Babet representing the party in the upper house.
A notice published by the Australian Electoral Commission said the party was deregistered on September 8.
The decision appeared to surprise Senator Babet, who at the time said the party had been deregistered for "administrative reasons" and would be re-registered ahead of the next election.
"This has actually happened before. We do it in between the elections to save on the administration, the party is not going anywhere. It's staying right where it is. The UAP is here forever," he said.
Mr Palmer accused Labor and the Liberals of "rigging the election" by supporting the party registration laws that he claimed were aimed at "eliminating competition".
"We don't believe the provisions of the Electoral Act are conducive to running a free and fair election as our Constitution demands," he said at the time.
The mining magnate spent more than $120 million campaigning at the last election with just Senator Babet elected.Â
Senator Babet is not facing re-election until 2028.
