Bees from 1533 hives have been destroyed between the NSW central and mid-north coasts as well as at Narrabri in the state's northwest, NSW Agriculture Minister Dugald Saunders says.
"It's a significant number of bees," he told AAP on Thursday.
Each hive contains anywhere from 10,000 to 30,000 bees, which means between 15 million and 45 million bees have been euthanised in an attempt to control the parasite since it was first detected near the Port of Newcastle on June 22.
There was an estimated 315,100 bee hives in NSW before the mite was detected.
"(Bees) do breed up again very quickly but it's about making sure you've got all of the people still wanting to stay in the industry after it's been decimated like this," the minister said.
Acting chief executive of the Australian Honey Bee Industry council Danny Le Feuvre said it's heartbreaking for those impacted.
"It's certainly devastating for the people involved...particularly the beekeepers who have spent alot of time and effort in building their businesses and building their hives up...and tending to them," Mr Le Feuvre told AAP.
"I can't imagine what they're feeling right now."
But he said the measures were necessary to protect the broader industry.
"What we're going through at the moment is the ...only way that we're going to be able to eradicate it," he said.
A ban restricting the movement of bees remains in place in NSW, with almond industry representatives calling for hives to be moved in time for pollination in August.
Mr Saunders said he was confident there would be a way of moving the hives eventually, but the lockdown remained in place for now.
"It's about making sure that we're not risking moving a devastating disease into an area that it isn't currently," Mr Saunders said.
"If we can have a good way of identifying where hives might come from to be part of the pollination, and if everyone feels safe around how that can work, then fantastic."
The minister remained optimistic the deadly mite could be contained, but said continuing co-operation from beekeepers was key.
"Everything we are doing is still working. We're finding more infected premises, as was expected. They are all linked, which is the good news part of it.
"We haven't seen an unexpected outbreak somewhere. We've just seen everything connected to a previous case, and that's important."
An expert from New Zealand arrived this week to give advice after the varroa mite infected that country 21 years ago.
Compensation for commercial beekeepers is still being worked out and counselling is being offered for those who need it.
"People are feeling a bit desperate, and everywhere we're looking now there's flooding as well. It just adds to the stress," Mr Saunders said.
3°C