A veteran of stage and screen, Humphries entertained generations with his satirical characters including the high camp of Dame Edna and the offensive cultural attache Sir Les Patterson.
Humphries died in April 2023 aged 89.
A sale of around 250 items from his personal collection will take place at Christie's in London on February 13.
The auction will include the diamante-encrusted Sydney Opera House spectacles worn by Dame Edna, which are estimated to sell for up to STG1,500 ($A2,986).
Barry Humphries became a staple of the UK comedy circuit after moving to London in 1959. (Joel Carrett/AAP PHOTOS)
Originally a drab Melbourne housewife satirising Australian suburbia, Dame Edna evolved into an increasingly boisterous and eccentric character, with "wisteria hue" hair and cat eye glasses.
Leading the sale is work by artist Charles Conder, whose painting Sand Dunes, Ambleteuse has a top estimate of STG300,000.
Elsewhere, his collection includes a first edition copy of Oscar Wilde's The Importance Of Being Earnest which is inscribed to Wilde's publisher and has a ceiling estimate of STG150,000.
The auction includes 19th century European and symbolist art, with Jean Delville's L'Oubli Des Passions estimated to sell for up to STG180,000, while Le Collier De Medailles by Fernand Khnopff has a top estimate of STG100,000.
The full pre-sale exhibition will be on public view from February 7-12.
Benedict Winter, associate director of private and iconic collections at Christie's London, said: "Barry Humphries is best remembered for his comedic genius, but behind his famous figure was a true polymath and connoisseur.
"His passion for collecting and his insatiable appetite for deepening his knowledge was the driving force behind the acquisition of these fascinating and very diverse works of art.
"This refined and engaging collection provides compelling insights into the private world of this very public performer."
Humphries became a staple of the British comedy circuit after moving from Australia to London in 1959 and appearing in West End shows such as Maggie May and Oliver!
He was among the leading members of the British comedy scene, alongside Dudley Moore, Alan Bennett and Spike Milligan while his talents extended into script writing and film producing.
In his stable of alter egos, he had the gentlemanly Sandy Stone, 1960s underground film-maker Martin Agrippa and sleazy trade union official Lance Boyle among many others.
