Luongo came out of international retirement and was named by new Socceroos coach Tony Popovic for the upcoming matches against China and Japan.
The two games are crucial for Australia's hopes of earning direct qualification for the 2026 World Cup in North America.
But Football Australia revealed on Sunday that Luongo won't be available for either international, citing an ankle injury suffered while with his Premier League club Ipswich Town.
The injury also ruled out Luongo from the Tractor Boys' 4-1 loss to West Ham United on the weekend.
In another blow for the Socceroos, Connor Metcalfe, who plays for Bundesliga outfit St Pauli, has also been ruled out with a leg injury.
No time frames have been set for the return of either Luongo or Metcalfe.
Luke Brattan (Macarthur FC) and Patrick Yazbek (Nashville SC) have been brought in to replace the injured duo.
Tony Popovic has been forced to make two changes to his extended 26-man — Subway Socceroos (@Socceroos) #Socceroos squad for our upcoming qualifiers.Massimo Luongo and Connor Metcalfe have been ruled out through injury, with the midfield duo replaced by Luke Brattan and Patrick Yazbek.📰 Details:… pic.twitter.com/CNh5Mm9yVsOctober 6, 2024
Yazbek earns a recall for the first time since making his international debut against Lebanon in March 2024, while Brattan joins the Socceroos' squad for the first time since 2018.
The Socceroos face China in Adelaide on Thursday before flying to Saitama for a tough clash with Japan.Â
Graham Arnold stepped down as Socceroos coach after Australia started this phase of World Cup qualifying with a 1-0 home loss to Bahrain and a 0-0 draw away to Indonesia.
Only the top two nations of the six-team group are guaranteed a spot at the World Cup, with the third-placed team forced into another phase of qualifying.
Japan currently sit top of Group C with two wins from as many outings.
Saudi Arabia are second with a win and a draw, while Bahrain (three points) and Indonesia (two) are third and fourth respectively.
Australia, with just one point to their name, sit fifth, above only China (zero).
Luongo won the 2015 Asian Cup's MVP award in Australia's triumphant tournament and last appeared for the Socceroos in November last year.
He was set to be among eight new faces from a squad that flopped badly against Bahrain and Indonesia.
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