Third-placed Atalanta lost 1-0 at home to Lazio on Sunday for their third straight league defeat.
That has seen Gian Piero Gasperini's team fall 10 points behind league leaders Inter Milan and left them looking nervously over their shoulders.
Atalanta are only two points ahead of Juventus after the Bianconeri drew 1-1 at Roma. Bologna can leapfrog Atalanta if they beat second-placed Napoli on Monday.
Lazio, who came into the match without a victory in Serie A in more than a month, moved to within three points of Atalanta.
Both teams were lacking in confidence and that perhaps explained the cagey first half.
Atalanta appeared reinvigorated after the break and had two quick chances, with Lazio goalkeeper Chrīstos Mandas making an extraordinary save to deny the league's top scorer Mateo Retegui at point-blank range.
However, it was Lazio who broke the deadlock nine minutes into the second half, through halftime substitute Gustav Isaksen.
Fisayo Dele-Bashiru nodded a long ball over the top down to Isaksen, who scuffed a shot past Marco Carnesecchi.
Roma's streak of seven straight league wins — with only one goal conceded — was ended by Juventus, in Igor Tudor's second match in charge of the Bianconeri.
It was an aggressive start from Juventus, and Roma goalkeeper Mile Svilar had to palm Nico González's header onto his right post.
Juventus took the lead shortly before halftime. Khéphren Thuram's cross was cleared by Zeki Çelik but only to the edge of the area where Manuel Locatelli volleyed it into the ground to bounce into the bottom left corner.
It was the first goal Roma had conceded in Serie A since March 2.
Claudio Ranieri's halftime substitution had an immediate impact as Eldor Shomurodov leveled three minutes after coming on, heading in the rebound from three yards after Michele Di Gregorio had parried Evan N'Dicka's attempt.
Roma moved to within three points of the top four.
Lecce were loudly jeered by their home fans despite rallying to draw 1-1 against Venezia in a relegation dogfight and ending a run of five straight defeats.
Federico Baschirotto headed in the equaliser for Lecce in the 65th after home defender Antonino Gallo had bundled into his own net at the start of the second half.
Lecce remained in the last position of safety in Serie A, two points above Empoli which drew 0-0 against Cagliari.
Venezia were in penultimate position, five points below Lecce.
Torino forward Che Adams had a penalty saved in a 1-1 draw at home to Hellas Verona.
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