The Australians have missed Tupou in two disappointing losses at home to South Africa, with the tighthead given time to grieve the death of his father.
But in the La Plata clash on Saturday (8am Sunday AEST) the Wallabies are hoping Tupou can make an early impact, with coach Joe Schmidt opting to start Allan Alaalatoa from the bench, with Angus Bell the run-on loosehead prop.
Veteran James Slipper wasn't considered after his late arrival into camp following the birth of his second daughter.
Winger Andrew Kellaway says the Wallabies combinations are benefiting from touring time together. (Darren England/AAP PHOTOS)
Estadio Jorge Luis Hirschi is expected to be heaving after their historic win over New Zealand to open the tournament, while home-town hero, former captain Agustin Creevy announced he would retire after the Test.
Named on the bench, the 39-year-old hooker will end a 19-year international career with a record 110 caps including 51 as skipper, and the only Puma to have played in four World Cups.
Young Wallabies prop Isaac Isaac Kailea, included in the reserves, said that the team missed Tupou against the Springboks.
"We sort of missed him in those two games against South Africa, and in particular around the scrum," said 24-year-old Kailea, set to play his fifth Test.
"He's such a big, literally, such a big asset in the scrum and around the field, whether he's got the ball in his hands or he's outside you for that little tip pass.
"He's got real good skill and he's such a powerful ball carrier.
"He's going to draw in eyes and a lot of attention to himself and I think he'll thrive off that.
"And I think he's really keen to get into the game as well, just speaking to him throughout the week."
On their last visit to Argentina in 2022 the tourists won their first Test in difficult circumstances with skipper Michael Hooper withdrawing on match eve for mental health reasons while playmaker Quade Cooper ruptured his achilles mid-game.
The Pumas roared back to take the second Test, while Argentina also won their only meeting last year before they went through to the World Cup semi-finals.
Pumas players celebrate their 2022 Test win over the Wallabies in San Juan. (AP PHOTO)
Veteran utility back Andrew Kellaway didn't play in 2022 under then coach Dave Rennie but said that the matches had been a talking point this trip.
"We talked about that quite a bit," he said.
"We got peppered with high balls ... to be frank, that's not dissimilar to the way South Africa plays so that's beneficial in terms of preparing for this game," the 28-year-old said.
"The challenge for us is to make sure we're on and we're prepared and we're certainly doing our best to do that.
"The focus has been almost exclusively on us this week, making sure we're getting the stuff right that we need to do on our side of the ball and hopefully that puts us in a position to win the game."
