Harry was pictured dropping the puck for Tomas Hertl from the San Jose Sharks and Vancouver Canucks' Quinn Hughes during a ceremonial face-off ahead of the whistle.
The duke joined his wife in the stands as they watched the Canucks win 3-1 over the Californian team.
The Invictus Games, the international multi-sport event for wounded, injured and sick armed forces personnel which the duke founded in 2014, will be held in Vancouver and Whistler in 2025.
Prince Harry and Meghan Markle joined crowds at the Vancouver Canucks and San Jose Sharks NHL game.
The couple appeared in public together for the first time at the Toronto Invictus Games in 2017.
Harry's grandmother, Queen Elizabeth II, made the ceremonial puck drop at a 2002 match, also between the Vancouver Canucks and San Jose Sharks, during her two-week Golden Jubilee tour of Canada.
It was the first NHL game the Queen had attended since 1951 when, still a princess, she and Prince Philip watched the Montreal Canadiens take on the New York Rangers.
The Sussexes' sporting outing comes a few days after Meghan's surprise arrival at the Variety Power Of Women event in Los Angeles to celebrate the accomplishments of women in entertainment.
The former Suits actress said she was "thrilled" to have returned to the entertainment industry with Archewell Productions and is "really proud" of the projects they have created so far, including docu-series Harry and Meghan and Heart Of Invictus.
"We have so many exciting things on the slate," she said, teasing future projects
"I can't wait until we can announce them but I'm just really proud of what we're creating."
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