The National Health Service said a 41-year-old Costa Rican woman died on Saturday from injuries sustained in the tragedy at the Jet Set discotheque during a a merengue concert at the Jet Set nightclub in Santo Domingo, where more than 200 were injured.
Hours earlier, Dominican Public Health Minister Victor Atallah said the death toll had risen from 221 to 225 after four people died between Friday night and early on Saturday morning.
Part of the walls, twisted iron, and remains of the nightclub's lighting are being guarded on the capital's seafront.
In front of the discotheque, a fence now blocks the view of the disaster area, where there have been fumigations.
Dominican President Luis Abinader said on Friday that there must be answers as to "what happened, why it happened, and how it happened".
The government announced a commission of national and international experts would to determine the cause of the accident early on Tuesday.
Zulinka Perez, daughter of merengue singer Rubby Pérez, who died in the accident, said she was suing the owners of the establishment for negligence.
Among those who died at Jet Set were former Major League Baseball pitcher Octavio Dotel, the governor of Montecristi, Nelsy Cruz, former baseball player Tony Blanco, a son of former Senate President and Minister of Public Works, Eduardo Estrella, and high-ranking bank executives.
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