The new piece at the zoo shows a gorilla lifting up the shutter to allow a number of birds to escape, while the eyes of other animals can be seen lurking in the darkness.
The artwork, which the artist has posted on his official Instagram account, appears to tie in to previous pieces, with animals scattered around London outside of their natural habitat.
The Zoological Society of London confirmed that it will protect the image with a perspex cover, and will leave the shutter down for the thousands of visitors to view on Tuesday.
It comes after Banksy's eighth piece, a rhino mounting a silver Nissan Micra with a traffic cone on its bonnet, was defaced by a man wearing a black balaclava just hours after it went up.
View this post on Instagram A post shared by Banksy (@banksy)
Banksy has been posting artwork to his Instagram every day since August 5, and it is unclear how long he will continue dotting works around London.
On Sunday, the artist depicted piranhas on a police box near the Central Criminal Court, known as the Old Bailey, in London.
A small crowd of people flocked to the fish tank-themed artwork on Monday, taking photos and selfies as workmen placed barriers around it. A spokesman for the City of London Corporation said it was looking at options to preserve it.
A design of swimming fish by Banksy has appeared on a police box in London. (AP PHOTO)
Other pieces unveiled last week included pelicans that appeared on the side of a fish shop in Walthamstow, east London, and a silhouette of a howling wolf that was painted on a satellite dish on a garage roof in south London.
The wolf design was seen taken down by men who carried it off on the same day it was revealed.
Banksy began his career spray-painting buildings in Bristol and has become one of the world's best-known artists. His work has sold for millions of dollars at auction, and past murals on outdoor sites have often been stolen or removed by building owners soon after going up.
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