Violence has resulted in the deaths of 117 women since January 2024, including 14 killed since the start of 2025.
"The women who have been killed are not statistics. They're individual lives," organiser Sherele Moody told AAP.
"Tens of thousands of women experience abuse in Australia every day."
Rallies will take place in 14 cities on Saturday, organised and funded by Australian Femicide Watch and the Red Heart Campaign.
They are intended to be memorials for the women lost, and a call for politicians to take more decisive action to combat violence against women.
"These rallies are also about giving voice to people impacted by femicide and a giant memorial for the 117 women killed," Ms Moody said.
"We will commemorate these women and show that their lives mattered.
"We are doing everything we can to wake Australia up."
Greens senator Dorinda Cox will attend the Perth rally and give a speech, intending to urge MPs to rise above politics to tackle the national emergency of femicide.
"We expect the prime minister to fix this and for him not to attend one of the rallies is telling, because this should be a national priority," she said.
"This is a state of emergency."
While Australian Femicide Watch documents the number of female deaths to violence, advocates say a national register is needed to track the extent of the issue.
"We don't have any good nationally harmonised data - we count the road toll but not the number of women who die in this country," Senator Cox said.
Rally organisers hope the demonstrations will put pressure on all political parties ahead of the upcoming federal election.
Men are encouraged to attend and be part of a supportive solution to the problem.
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